| 质粒名称: | pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 103865 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 9360 bp |
| 完整质粒大小: | 12606 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PspCas13b |
| 物种来源: | Prevotella sp. P5-125 |
| 插入大小: | 3270 bp |
| 基因突变: | Contains the H133A and H1058A mutations which inactivate the HEPN RNase domains of Cas13b. |
| GenBank编号 | WP_044065294 |
| 启动子 | EF1a |
| 标签/融合蛋白: |
HIV NES（插入件上的C端子）
3xHA（插入件上的C端子）
|
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | EF1a-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A was a gift from Feng Zhang (Addgene plasmid # 103865 ; http://n2t.net/addgene:103865 ; RRID:Addgene_103865) |
| 参考文献部分: | RNA editing with CRISPR-Cas13. Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. 10.1126/science.aaq0180 PubMed 29070703 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A 12607 bp ds-DNA circular SYN 03-MAY-2019
DEFINITION Catalytically inactive PSPCas13b. RNase activity is inactivated by
mutation of catalytic histidine residues in the HEPN domains
(H133A/H1058A)..
ACCESSION .
VERSION .
KEYWORDS pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12607)
AUTHORS Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung
J, Zhang F
TITLE RNA editing with CRISPR-Cas13.
JOURNAL Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
PUBMED 29070703
REFERENCE 2 (bases 1 to 12607)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 3, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12607
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2682..3860
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2913..3851
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3808..3828
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 7155..7181
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
CDS 7191..7271
/codon_start=1
/product="three tandem HA epitope tags"
/label=3xHA
/translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
misc_feature 7299..7887
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7352..7372)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7770..7781)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(7890..7906)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7891..7907)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 8412..8592
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(8618..8635)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8624..8848
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 8894..9322
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8981..9000)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9191..9212
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9331..9351)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9336..9665
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9516..9651
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9578..9597
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 9713..9760
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 9779..10153
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 10283..10404
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10320..10339)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10374..10393
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10453..10469)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10453..10469)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10466..10488)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10477..10493
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10501..10531)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10546..10567
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10684..10701)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10855..11443)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10935..10954)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11614..12474)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12237..12256
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12475..12579)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
3001 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
3061 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
3121 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
3181 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
3241 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
3301 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
3361 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
3421 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
3481 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
3541 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
3601 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
3661 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
3721 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
3781 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
3841 ttccatttca ggtgtcgtga cgtacggcca ccatgaacat ccccgctctg gtggaaaacc
3901 agaagaagta ctttggcacc tacagcgtga tggccatgct gaacgctcag accgtgctgg
3961 accacatcca gaaggtggcc gatattgagg gcgagcagaa cgagaacaac gagaatctgt
4021 ggtttcaccc cgtgatgagc cacctgtaca acgccaagaa cggctacgac aagcagcccg
4081 agaaaaccat gttcatcatc gagcggctgc agagctactt cccattcctg aagatcatgg
4141 ccgagaacca gagagagtac agcaacggca agtacaagca gaaccgcgtg gaagtgaaca
4201 gcaacgacat cttcgaggtg ctgaagcgcg ccttcggcgt gctgaagatg tacagggacc
4261 tgaccaacgc atacaagacc tacgaggaaa agctgaacga cggctgcgag ttcctgacca
4321 gcacagagca acctctgagc ggcatgatca acaactacta cacagtggcc ctgcggaaca
4381 tgaacgagag atacggctac aagacagagg acctggcctt catccaggac aagcggttca
4441 agttcgtgaa ggacgcctac ggcaagaaaa agtcccaagt gaataccgga ttcttcctga
4501 gcctgcagga ctacaacggc gacacacaga agaagctgca cctgagcgga gtgggaatcg
4561 ccctgctgat ctgcctgttc ctggacaagc agtacatcaa catctttctg agcaggctgc
4621 ccatcttctc cagctacaat gcccagagcg aggaacggcg gatcatcatc agatccttcg
4681 gcatcaacag catcaagctg cccaaggacc ggatccacag cgagaagtcc aacaagagcg
4741 tggccatgga tatgctcaac gaagtgaagc ggtgccccga cgagctgttc acaacactgt
4801 ctgccgagaa gcagtcccgg ttcagaatca tcagcgacga ccacaatgaa gtgctgatga
4861 agcggagcag cgacagattc gtgcctctgc tgctgcagta tatcgattac ggcaagctgt
4921 tcgaccacat caggttccac gtgaacatgg gcaagctgag atacctgctg aaggccgaca
4981 agacctgcat cgacggccag accagagtca gagtgatcga gcagcccctg aacggcttcg
5041 gcagactgga agaggccgag acaatgcgga agcaagagaa cggcaccttc ggcaacagcg
5101 gcatccggat cagagacttc gagaacatga agcgggacga cgccaatcct gccaactatc
5161 cctacatcgt ggacacctac acacactaca tcctggaaaa caacaaggtc gagatgttta
5221 tcaacgacaa agaggacagc gccccactgc tgcccgtgat cgaggatgat agatacgtgg
5281 tcaagacaat ccccagctgc cggatgagca ccctggaaat tccagccatg gccttccaca
5341 tgtttctgtt cggcagcaag aaaaccgaga agctgatcgt ggacgtgcac aaccggtaca
5401 agagactgtt ccaggccatg cagaaagaag aagtgaccgc cgagaatatc gccagcttcg
5461 gaatcgccga gagcgacctg cctcagaaga tcctggatct gatcagcggc aatgcccacg
5521 gcaaggatgt ggacgccttc atcagactga ccgtggacga catgctgacc gacaccgagc
5581 ggagaatcaa gagattcaag gacgaccgga agtccattcg gagcgccgac aacaagatgg
5641 gaaagagagg cttcaagcag atctccacag gcaagctggc cgacttcctg gccaaggaca
5701 tcgtgctgtt tcagcccagc gtgaacgatg gcgagaacaa gatcaccggc ctgaactacc
5761 ggatcatgca gagcgccatt gccgtgtacg atagcggcga cgattacgag gccaagcagc
5821 agttcaagct gatgttcgag aaggcccggc tgatcggcaa gggcacaaca gagcctcatc
5881 catttctgta caaggtgttc gcccgcagca tccccgccaa tgccgtcgag ttctacgagc
5941 gctacctgat cgagcggaag ttctacctga ccggcctgtc caacgagatc aagaaaggca
6001 acagagtgga tgtgcccttc atccggcggg accagaacaa gtggaaaaca cccgccatga
6061 agaccctggg cagaatctac agcgaggatc tgcccgtgga actgcccaga cagatgttcg
6121 acaatgagat caagtcccac ctgaagtccc tgccacagat ggaaggcatc gacttcaaca
6181 atgccaacgt gacctatctg atcgccgagt acatgaagag agtgctggac gacgacttcc
6241 agaccttcta ccagtggaac cgcaactacc ggtacatgga catgcttaag ggcgagtacg
6301 acagaaaggg ctccctgcag cactgcttca ccagcgtgga agagagagaa ggcctctgga
6361 aagagcgggc ctccagaaca gagcggtaca gaaagcaggc cagcaacaag atccgcagca
6421 accggcagat gagaaacgcc agcagcgaag agatcgagac aatcctggat aagcggctga
6481 gcaacagccg gaacgagtac cagaaaagcg agaaagtgat ccggcgctac agagtgcagg
6541 atgccctgct gtttctgctg gccaaaaaga ccctgaccga actggccgat ttcgacggcg
6601 agaggttcaa actgaaagaa atcatgcccg acgccgagaa gggaatcctg agcgagatca
6661 tgcccatgag cttcaccttc gagaaaggcg gcaagaagta caccatcacc agcgagggca
6721 tgaagctgaa gaactacggc gacttctttg tgctggctag cgacaagagg atcggcaacc
6781 tgctggaact cgtgggcagc gacatcgtgt ccaaagagga tatcatggaa gagttcaaca
6841 aatacgacca gtgcaggccc gagatcagct ccatcgtgtt caacctggaa aagtgggcct
6901 tcgacacata ccccgagctg tctgccagag tggaccggga agagaaggtg gacttcaaga
6961 gcatcctgaa aatcctgctg aacaacaaga acatcaacaa agagcagagc gacatcctgc
7021 ggaagatccg gaacgccttc gatgcaaaca attaccccga caaaggcgtg gtggaaatca
7081 aggccctgcc tgagatcgcc atgagcatca agaaggcctt tggggagtac gccatcatga
7141 agggatccct tcaactgcct ccacttgaaa gactgacact gggtagttcc tacccatacg
7201 atgttccaga ttacgcttat ccctacgacg tgcctgatta tgcataccca tatgatgtcc
7261 ccgactatgc ctaagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
7321 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
7381 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
7441 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
7501 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
7561 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
7621 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
7681 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
7741 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
7801 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
7861 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
7921 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
7981 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
8041 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
8101 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
8161 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
8221 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
8281 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
8341 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
8401 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
8461 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
8521 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
8581 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
8641 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
8701 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
8761 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
8821 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
8881 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
8941 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
9001 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
9061 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
9121 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
9181 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
9241 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
9301 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
9361 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
9421 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
9481 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
9541 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
9601 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
9661 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
9721 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
9781 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
9841 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
9901 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
9961 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
10021 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
10081 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
10141 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
10201 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
10261 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
10321 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
10381 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
10441 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
10501 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
10561 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
10621 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
10681 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
10741 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
10801 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
10861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
10921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
10981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
11041 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
11101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
11161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
11221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
11281 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
11341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
11401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
11461 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
11521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
11581 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
11641 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
11701 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
11761 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
11821 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
11881 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
11941 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
12001 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
12061 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
12121 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
12181 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
12241 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
12301 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
12361 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
12421 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
12481 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
12541 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
12601 acctgac
//
